1 edition of Methods of extracting compounds related to the steroid hormones from human urine found in the catalog.
Methods of extracting compounds related to the steroid hormones from human urine
|Statement||by N. H. Callow [et al.].|
|Contributions||Callow, N. H.|
|The Physical Object|
|Pagination||p. 76-98 ;|
|Number of Pages||98|
These methods measure the levels of steroids by a physical method of separation (LC or GC), based on molecular size, followed by mass spectrometry (mass/charge analysis). 4,5,12 Because the LC and GC methods separate and differentiate the active steroid from its metabolites, they provide the most accurate and precise assessment of the steroids. Solid-phase extraction is a convenient way to extract steroids from urine, but there are large differences in extraction recovery of various columns. The two most efficient columns are Bakerbond C18 and Isolute by:
A rapid and sensitive reversed-phase liquid chromatographic method (RP-LC) with UV detection has been developed for the determination of free cortisol, cortisone and corticosterone in human urine. The assay was performed after a solid-phase extraction procedure (SPE) with dexamethasone as the internal tographic separation was carried out on a Nucleosil C 18 analytical Cited by: 1. Adrenocorticotropic hormone 2. Follicle-stimulating hormone 3. Growth hormone 4. Luteinizing hormone 5. Thyroid stimulating hormone 6. Melanocyte-stimulating hormone 7. Prolactin [All Friendly Girls Like To Meet People].
Background: Steroid hormones belong to a group of low-molecular weight compounds which are responsible for maintenance of various body functions, thus, the Cited by: 2. Scientists have so far isolated and specified around as against the estimated figure of more than two thousand elements and compounds in human urine. 4) Biochemistry. Urine basically consists of 95% water and 5% substances like minerals, vitamins, proteins, enzymes, hormones, antibodies and .
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Due to the need to identify and quantify these compounds, a new fully automated method was developed for the simultaneous determination of the two forms of several steroid hormones (free and conjugated) in different water matrixes and in by: Method Validation and Additional Performance Data 53 Figure 8.
Relation between analyte method recoveries and isotope- dilution standard absolute recoveries in percent (%) in spiked replicates of reagent water, surface water, secondary wastewater effluent, and primary wastewater effluent validation by: Due to the need to identify and quantify these compounds, a new fully automated method was developed for the simultaneous determination of the two forms of several steroid hormones (free and conjugated) in different water matrixes and in urine.
The method is based on online solid phase extraction coupled with liquid chromatography and tandem mass spectrometry Cited by: Steroid hormones are crucial substances that mediate a wide range of vital physiological functions of the body.
Determination of the levels of steroid hormones plays an important role in understanding the mechanism of the steroid hormone-related diseases. In this study, we present a novel targeted metabolic profiling method based on the introduction of an easily protonated stable Cited by: The fragmentation behaviors, DPIs, elemental composition and double-bond equivalent were then simultaneously utilized for systematical characterization of steroid hormones in human urine.
Consequently, fragmentation pathways and DPIs for all types of steroid hormones were comprehensively : Yuanyuan Zheng, Hongzhi Zhao, Lin Zhu, Zongwei Cai.
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Rule G, Henion J () High-throughput sample preparation and analysis using well membrane solid-phase extraction and liquid chromatography – tandem mass spectrometry for the determination of steroids in human urine.
Soc. Mass Spectrom. 10; – Google ScholarCited by: 3 General Methods for the Extraction, Purification, and Measurement after addition to the matrix, be distributed (e.g.
bound to any protein, etc.) in the same way as the analyte. at C-3 have previously been isolated from human urine; their precursors, however, have been steroid hormones, such as progesterone and corti- costerone, which lacked the 17cu-hydroxyl group.
The presence in urine of the &-hydroxyallopregnane compounds described in this investigation. To extend the possibility of implementing surface-enhanced infrared absorption (SEIRA) for biological applications, the detection and analysis of the steroid hormone cortisol was demonstrated.
The use of positive or negative ionization mode was evaluated by Cuzzola et al. in the only report in literature that describes the measurement of the same five steroids in human urine in a single analytical run. In this paper, the negative mode was selected for further fragmentation and quantification of the by: Steroid hormone biosynthesis pathway with some steroid metabolites in the human body.
The four steroid classes are progestogens (yellow), corticosteroids (green), androgens (blue) and Cited by: 3. Methods in Hormone Research, Volume II: Bioassay focuses on the detailed and critical account of bioassay methods, including electrolyte and carbohydrate methods, hormones, and assays.
The selection first offers information on the statistical methods and steroid hormones and related Book Edition: 1. The elaborated MEKC method was developed to estimate the levels of seven steroid hormones in human urine samples from amateur weight-lifters using hormonal doping (13 persons), amateur weight-lifters not using hormonal doping (three persons) and healthy volunteers (five persons).Cited by: 5.
THE MALE SEX HORMONES OF HUMAN URINE AND BLOOD BY D. ROY McCULLAGH AND W. OSBORN presented in this paper to prove the existence of such a compound, and to show it to be insoluble in dibutyl ether. EXPERIMENTAL A method is described for the extraction and assay of an- drogens in blood. The level of a steroid hormone, including estrogen, in the stratum corneum of the skin is assayed by LC-MS analysis of a steroid hormone in the stratum corneum of the skin collected by a tape stripping method.
This allows the concentration in the skin of the steroid hormone, including estrogen which has an important effect on the state of the skin, to be easily by: 6. SIMPLE, EFFICIENT AND SIMULTANEOUS DETERMINATION OF ANABOLIC STEROIDS IN HUMAN URINE Pages with reference to book, From To S.J.
Khurshid, M. Riaz, S. Ahmed (Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad. The method requires only ml of urine and approximately 60 urine samples can be quantitatively analyzed per week. host of other hormone-related disorders. 2 Cited by: The lower limit of quantitation for each estrogen is ng/ mL urine sample (2 pg on column), with the percent recovery of a known added amount of compound (accuracy) of 96−% and an overall precision, including the hydrolysis, extraction, and derivatization steps, of 1−5% relative standard deviation (RSD) for samples prepared Cited by: Free steroids are available for action in target cells and also for metabolism in peripheral tissues.
Thus, SHBG and CBG play an important role in regulating the availability of steroid hormones for biologic action and clearance. Table 3. Binding distribution of principle endogenous steroid hormones in normal women during the menstrual cycle*. Congenital adrenal hyperplasia (CAH) is another clinical disease that affects the levels of steroid hormones that are produced by the adrenal cortex.
Congenital adrenal hyperplasia is a group of inherited diseases that result in defective activity of one of five enzymes in the adrenal by: This method suffers from considerable interferences from the effects of drugs (spironolactone, phenytoin, antibiotics) and ketonecontaining compounds.
In the UK, urine steroid quantitation relied upon the estimation of oxogenic steroids (hydroxyl and hydroxyl or oxo group) and oxosteroids using the Zimmerman reaction as an approximate guide to adrenal corticosteroid and. The method involves a fully automated sample preparation including enzyme treatment, addition of internal standards and solid phase extraction followed by analysis by liquid chromatography–tandem mass spectrometry (LC–MS-MS) using electrospray ionization with adduct formation for two compounds.
Urine samples from forensic cases were Cited by: 4.